Wei Wang

Institute of Life Science, Jiyang College of Zhejiang A&F University, Zhuji, 311800
Author    Correspondence author
International Journal of Molecular Medical Science, 2023, Vol. 13, No. 3   doi: 10.5376/ijmms.2023.13.0003
Received: 07 Sep., 2023    Accepted: 25 Sep., 2022    Published: 18 Oct., 2023

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

 

Gene editing technology is a precise modification technique targeted at DNA sequences, which has been widely applied in biomedical research, agriculture, and animal husbandry, among other fields. Gene editing technologies mainly include nucleases-based techniques, CRISPR-Cas system-based techniques, transcription activator-based techniques, and chemical modification-based techniques. Although these techniques employ different tools and methods for DNA sequence modification, their principles involve targeted cleavage of DNA sequences to induce double-strand breaks, followed by repair through intrinsic DNA repair mechanisms to achieve modification of the target genes. This review introduces the principles and applications of different types of gene editing technologies, analyzes their advantages and challenges, and discusses the future prospects of gene editing technology, providing valuable references and guidance for its research and applications.

Gene editing technology; CRISPR-Cas9; DNA repair; Application prospects